Plasmid

Part:BBa_K5302021:Design

Designed by: Xinyuan Shi   Group: iGEM24_USTC   (2024-10-01)


pBBR-OmpA-miniZ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4025
    Illegal XbaI site found at 3140
    Illegal PstI site found at 1967
    Illegal PstI site found at 3128
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4025
    Illegal PstI site found at 1967
    Illegal PstI site found at 3128
    Illegal NotI site found at 1008
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4025
    Illegal BglII site found at 1754
    Illegal BamHI site found at 3146
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4025
    Illegal XbaI site found at 3140
    Illegal PstI site found at 1967
    Illegal PstI site found at 3128
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4025
    Illegal XbaI site found at 3140
    Illegal PstI site found at 1967
    Illegal PstI site found at 3128
    Illegal NgoMIV site found at 2418
    Illegal NgoMIV site found at 2701
    Illegal NgoMIV site found at 4683
    Illegal AgeI site found at 4523
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1330
    Illegal SapI.rc site found at 2267
    Illegal SapI.rc site found at 2477


Design Notes

Please be advised that all listed genes have undergone codon optimization for our chassis strain, Escherichia coli Nissle 1917 (EcN). For those considering alternative chassis organisms, it is recommended to reassess the necessity of codon optimization.


Source

OmpA (Outer membrane protein A) from Escherichia coli str. K-12 substr. MG1655. miniZ from rational design.

References